Raf-1 (phospho Ser621) Polyclonal Antibody

Sizes: 50μL, 100μL

Catalogue Numbers: RA29218-50, RA29218-100

Citations, Manuals and MSDS Available upon request.

Background: Raf-1 proto-oncogene, serine/threonine kinase (RAF1) Homo sapiens This gene is the cellular homolog of viral raf gene (v-raf) . The encoded protein is a MAP kinase kinase kinase (MAP3K), which functions downstream of the Ras family of membrane associated GTPases to which it binds directly. Once activated, the cellular RAF1 protein can phosphorylate to activate the dual specificity protein kinases MEK1 and MEK2, which in turn phosphorylate to activate the serine/threonine specific protein kinases, ERK1 and ERK2. Activated ERKs are pleiotropic effectors of cell physiology and play an important role in the control of gene expression involved in the cell division cycle, apoptosis, cell differentiation and cell migration. Mutations in this gene are associated with Noonan syndrome 5 and LEOPARD syndrome 2. [provided by RefSeq, Jul 2008],

Condition: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Storage Instructions: -20°C/1 year

Recommended dilutions: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/5000. Not yet tested in other applications.

Alternative Names: RAF1; RAF; RAF proto-oncogene serine/threonine-protein kinase; Proto-oncogene c-RAF; cRaf; Raf-1

Applications: WB; IHC-p; IF/ICC; ELISA

Species Cross-Reactivity: Human; Mouse; Rat; Monkey

GeneID (Human): 5894

Protein MW (KDa): 73

SWISS: P04049

Source: Rabbit

Research Use Only